Fluorometric determination of prostaglandins and thromboxane in plasma by high-performance liquid chromatography.

is a useful method. Although there have been some reports9~11) on the HPLC analysis of PGs and TX using 9-anthryldiazomethane (ADAM), these methods have less sensitivity than HPLC for the quantitative determination of PGs and TX in human plasma. The purpose of this investigation was to establish an HPLC method for the determination of PGs and TX in biological samples. For the analysis of PGs and TX at higher sensitivities, we investigated the optimal detection method for ADAM derivatives of PGs and TX. Previous reports have not paid attention to the Raman spectrum of the eluent in the HPLC system in the fluorometric analysis of